Lysis of Human Monocytic Leukemia Cells by Extracellular Adenosine Triphosphate: Mechanism and Characterization
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چکیده
The present study shows that extracellular adenosine triphosphate (ATP) has the capacity to mediate dose-dependent lysis of the monocytic leukemia cell line THP-1. The lysis, assessed by 5‘Cr release, was found to be selective for ATP, because adenosine diphosphate (ADP) or other nucleotides were less effective in their ability to lyse the cells. The amount of “Cr released was particularly enhanced by the stimulation of the cells with 1,000 U/mL of interferon gamma (IFN-7) for 3 days, and the sensitivity was time and dose dependent. Analysis of the mechanism of lysis indicated that the fully ionized form, A T P , me-
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تاریخ انتشار 2003